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1-80402: Custom Peptide
Antibody Production |
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Introduction: |
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This package is designed for
generating polyclonal antibodies against a
synthesized pepide. |
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What is included with this
package: |
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Service |
Details |
Price |
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Peptide Antibody
Production in Rabbits |
-Synthesis of up to a
18aa peptide
-Conjugation of the
peptide to a carrier
protein
-14 week antibody
production schedule,
which includes 2 rabbits
and provides 1
pre-immune serum
collection, 4
immunizations, and 4
production bleeds (at
roughly 20-25ml serum
each) per animal, for a
total of approximately
160-200ml of polyclonal
anti-sera. |
$1095.00 |
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Peptide Antibody
Production in Chickens |
-Synthesis of up to a
18aa peptide
-Conjugation of the
peptide to a carrier
protein
-14 week antibody
production schedule,
which includes 2
chickens and provides 1
pre-immune serum
collection, 4
immunizations, 4 test
bleeds (at roughly 1ml
serum each) and 4 egg
collection periods per
animal. Each chicken
will normally produce 2
to 3 eggs per week, with
each yolk providing
approximately 15ml of
antibodies, for a total
of approximately
160-200ml of antibody. |
$1095.00 |
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Peptide Antibody
Production in Goats |
-Synthesis of up to a
18aa peptide
-Conjugation of the
peptide to a carrier
protein
-14 week antibody
production schedule in 1
goat, which provides 1
pre-immune serum
collection, 4
immunizations, and 4
production bleeds (at
roughly 250ml serum
each) for a total of
approximately 1000ml of
polyclonal anti-sera. |
$1495.00 |
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Peptide Synthesis |
Cost per each additional
peptide residue over the
18 residues included
with the package. |
$25.00 |
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Guidelines: |
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-Antigen Design Assistance is
included at no extra charge
-Typically, 45-55mg of peptide
will be synthesized (at immunograde purity)
-Customers will receive all
peptide that is not used for the
project.
-Generally, it is preferable to
add a cysteine to the N or C
terminal of the peptide (if no
internal cysteines are present
in the sequence) for conjugation
to the carrier protein. |
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Immunochemistry and
Additional Services: |
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Service |
Details |
Price |
| Sodium Azide |
Preservative for the
antiserum |
No Charge |
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| Western Blot |
Assay of purified
antiserum against
lysates |
No charge |
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| Affinity Purification |
Affinity Purification of
40 ml of serum or 15 ml of egg yoke. |
No Charge |
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| Exsanguination |
Terminal Bleed for
Rabbits. Provides
approximately 50ml of
polyclonal antiserum per
animal. |
$75.00 |
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| ELISA |
On 1 bleed date for 2
rabbits/chickens or 1
Goat |
$50.00 |
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| IgG Purification |
Purification of IgG from
25ml of serum |
$280.00 |
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| IgY Purification |
Purification of IgY from
25ml of egg yolk |
$420.00 |
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| 4 Week Extension |
4 Week Extension for
Rabbits or Chickens.
Provides 1 immunization
and 2 bleeds/egg
collections per animal
for approximately
80-100ml of polyclonal
antiserum. |
$150.00 |
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| 4 Week Extension-Goat |
4 Week Extension for
Goats. Provides 1
immunization and 2
bleeds, allowing for the
collection of
approximately 500ml of
polyclonal antiserum. |
$350.00 |
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| Exsanguination |
Terminal Bleed for
Rabbits. Provides
approximately 50ml of
polyclonal antiserum per
animal. |
$75.00 |
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| ELISA |
On 1 bleed date for 2
rabbits/chickens or 1
Goat |
$50.00 |
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