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Introduction: |
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This package is designed for
producing and purification of
3 mg recombinant protein in
E. Coli Expression System. |
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What is included with this
package: |
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Phases |
Details |
Cost |
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Phase 1:
Construct Generation: |
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Clone coding
sequence into
selected
expression vectors
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The expression
construct will be
fully sequence and
frame
verified
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Preparation of
plasmid
DNA
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1 week,
$500 |
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Phase 2:
Fusion |
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Introduce plasmid
DNA into
selected bacterial
of
over-expressing
strain cells.
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Small scale
expression and
purification study
assessed by SDS-PAGE
or Western blot
analysis
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Estimation of
protein yield.
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1.5 weeks,
$800 |
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Phase 3:
Screening |
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Large-scale growth
of over-expressing
strain
Cells followed by
harvesting
procedures.
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Protein typically
purified from
cleared lysate via
purification tag.
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SDS-PAGE or Western
blot analysis of
the purified protein.
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Determination of
protein
concentration.
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Delivery of purified
and ready-to-use protein.
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1.5 weeks,
$500 |
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Total |
3 mg purified
recombinant protein
production in E. coli |
4-5
weeks ($1,800) |
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Additional Options |
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Refill orders |
each additional
milligram protein |
1 week
$600/mg |
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