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1-30401: Custom
siRNA Library Construction in Lentiviral
Expression System |
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Introduction: |
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This package is designed for
constructing siRNA library
targeting 5,000 - 15,000 genes in
lentiviral expression
system. |
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What is included with this
package: |
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Phases |
Details |
Cost |
Phase 1:
siRNA Preparation: |
- Design optimal
siRNAs for 5,000 -
15,000 genes of your
choice, or you
provide your own
siRNA sequences.
- Synthesize
and purify siRNAs
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2 weeks
($1,500) |
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Phase 2:
Cloning |
- Clone the siRNA
into an CHI
Scientific
lentivector of your
choice—choose from
any of our siRNA
expression vectors
with the
fluorescence marker,
antibiotic
selection.
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1.5 weeks
($1,800) |
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Phase 3:
Squencing |
- Sequence a
representative
number of clones to
confirm sequences.
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1.5 weeks
($1,500) |
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Phase 4:
Packaging |
- Package
the library into
lentiviral
particles.
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2-3
weeks
($3,000) |
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Phase 5:
Titling and Delivery |
- Titling the
concentration of
packaged library
- Deliver the
packaged library
particles with
library plasmid
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1 week
($1,200) |
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Total |
- siTarget™ siRNAs
lentiviral
library for 5,000 -
15,000 genes
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7-9 weeks
($9,000) |
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