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Recombinant Ovine Leptin

Synonyms       OB Protein, Obesity Protein, OBS, Obesity factor. 
Introduction       A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. 
Description      Leptin Ovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.
The Leptin is purified by proprietary chromatographic techniques. 
Source      Escherichia Coli. 
Physical Appearance       Sterile Filtered White lyophilized (freeze-dried) powder. 
Formulation      The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. 
Solubility    It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 
Stability       Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. 
Purity       Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE. 
Amino acid sequence    The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg. 
Biological Activity      Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. 
Protein content       Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC,using calibrated solution of Leptin Ovine as a Reference Standard. 
Usage       CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 

Cat No. Description Unit Price Order
7-01156 Recombinant Ovine Leptin 20 µg $ 103.17 
7-01157 Recombinant Ovine Leptin 100 µg $ 268.25 
7-01158 Recombinant Ovine Leptin 1 mg $ 1809.52 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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