Synonyms Urokinase, Abbokinase, Urokinase-type Plasminogen Activator,uPA, EC 3.4.21.73, UK.
Introduction Urokinase (UK) is a serine protease, which is one of biological plasminogen activators.
It is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing.
It can be obtained from human urine or kidney cell culture.
Description Urokinase is a two-chain glycoprotein containing 411 amino acids with 12 disulfide bonds. Its molecular weight is 54,000 Dalton.
Source Human urine.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The Urokinase was lyophilized from a concentrated (1mg/ml) solution containing phosphate buffer.
Solubility It is recommended to reconstitute the lyophilized Urokinase in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Urokinase although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution Urokinase should be stored at 4℃ between 2-7 days and for future use below -18℃.
Please prevent freeze-thaw cycles.
Purity Greater than 90.0% as determined by SDS-PAGE.
Specific Activity 83,700IU/mg.
Activity 1nM UK will cause a change in absorbance of 0.001 at 405nm in 1 minute at R/T in 100 ul 0.05M Tris-HCl, 0.1M NaCl, pH 7.4, using S2444 (0.6mM) as the substrate.
Contaminants Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
References 1. Title:Transient ?-catenin stabilization modifies lineage output from human thymic CD34+CD1a– progenitors.
Publication:Published online before print December 1, 2009, doi: 10.1189/jlb.0509344 March 2010 Journal of Leukocyte Biology vol. 87 no. 3 405-414 .
2.Title:Activation of human pro-urokinase by unrelated proteases secreted by Pseudomonas aeruginosa.
Publication:Received 26 November 2009/18 March 2010; accepted 2
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.