Synonyms Avidin, AVD, AVID.
Introduction Avidin is a tetrameric protein of 4 identical subunits (homotetramer) each of which can bind to biotin with a high degree of affinity and specificity. Avidin molecular weight in its tetrameric form is estimated to be between 66-69 kDa. Avidin is produced in the oviducts of birds, reptiles and amphibians and is subsequently deposited in the whites of their eggs. In the chicken egg white, avidin makes up roughly 0.05% of total protein (approximately 1.8 mg per egg). 10% of Avidin’s molecular weight is ascribed to carbohydrate content which is composed of four to five mannose and three N-acetylglucosamine residues. Avidin has at least three distinctive oligosaccharide structural type which are similar in structure and composition. The dissociation constant (KD) of avidin is approximately 10-15M, making it one of the strongest known non-covalent bonds.
Description Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containg 4 subunits each with a binding site for biotin.
The Avidin is purified by affinity chromatographic techniques.
The purification procedure ensures minimal contamination by other proteins or DNA.
The resulting high activity and purity of the product gives very low non-specific binding (NSB).
Source Hen's egg white.
Physical Appearance Sterile Filtered white lyophilized powder.
Solubility It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ-cm H2O not less than 100µg/ml or more than 10mg/ml solutions.
Stability Avidin should be stored at 4°C.
Application Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
Activity 11-15 units/mg protein, 1 unit binds 1µg biotin.
Purity Greater than 98.0% as determined by SDS-PAGE.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.