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Human Breast PrimaCell™: Normal Mammary Epithelial Cells

The Human Breast PrimaCell™: Normal Mammary Epithelial Cells is for rapid (7-10 days) isolation and growth of normal human mammary epithelial cells from newborn or adult human breast tissues. This system features an optimal condition of tissue dissociation system, Breast OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Human Breast PrimaCell™ System (Cat No. 2-96023):
Human Breast PrimaCell™: Normal Mammary Epithelial Cells Protocols
Human Breast Tissue Preparation Buffer: Normal Mammary Epithelial Cells
Human Breast OptiTDS™: Tissue Dissociation System
Human Breast FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Human Breast PrimaCell™: Normal Mammary Epithelial Cells Growth Medium
Human Breast PrimaCell™: Normal Mammary Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Miao J, Fa Y, Gu B, Zhu W, Zou S. Taurine attenuates lipopolysaccharide-induced disfunction in mouse mammary epithelial cells. Cytokine. 2012 Jul;59(1):35-40. Epub 2012 May 10.
[2] Fedrowitz M, Hass R, Bertram C, Loscher W. Salivary alpha-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells. J Exp Clin Cancer Res. 2011 Oct 25;30(1):102.
[3] Bouchet BP, Puisieux A, Galmarini CM. βIII-Tubulin is required for interphase microtubule dynamics in untransformed human mammary epithelial cells. Eur J Cell Biol. 2011 Oct;90(10):872-8. Epub 2011 Aug 4.

Cat No. Description Unit Price Order
2-96023 Human Breast PrimaCell™: Normal Mammary Epithelial Cells Kit $ 1228.50 
4-28023 Human Breast OptiTDS™: Tissue Dissociation System Kit $ 274.05 
7-15020 Human Breast FibrOut™: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 302.40 
9-46023 Human Breast PrimaCell™: Normal Mammary Epithelial Primary Cells Growth Medium 5 x 100 ml $ 151.20 
9-47023 Human Breast PrimaCell™: Normal Mammary Epithelial Primary Cells Growth Supplements with Serum (for 500 ml medium) set $ 321.30 
9-80023 Human Breast Tissue Preparation Buffer: Normal Mammary Epithelial Primary Cells 1 x 100 ml $ 160.65 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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