The Rat Airway PrimaCell™3: Normal Bronchial and Tracheal Fibroblasts is for rapid (7-10 days) isolation and growth of normal rat bronchial fibroblasts from newborn or adult rat airway tissues. This system features an optimal condition of tissue dissociation system, Airway OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium.

Rat Airway PrimaCell™3 System (Cat No. 2-82503):
Rat Airway PrimaCell™3 Normal Bronchial and Tracheal Fibroblasts Protocols
Rat Airway Tissue Preparation Buffer 3: Normal Bronchial and Tracheal Fibroblasts
Rat Airway OptiTDS™3: Tissue Dissociation System
Rat Airway PrimaCell™3: Normal Bronchial and Tracheal Fibroblasts Growth Medium
Rat Airway PrimaCell™3: Normal Bronchial and Tracheal Fibroblasts Growth Supplements with Serum (for 500 ml medium)

References:

[1] Nonaka M, Pawankar R, Saji F, Yagi T. Distinct expression of RANTES and GM-CSF by lipopolysaccharide in human nasal fibroblasts but not in other airway fibroblasts. Int Arch Allergy Immunol. 1999 Aug;119(4):314-21.
[2] Nam YH, Lee SK, Sammut D, Davies DE, Howarth PH. Preliminary study of the cellular characteristics of primary bronchial fibroblasts in patients with asthma: expression of alpha-smooth muscle actin, fibronectin containing extra type III domain A, and smoothelin. J Investig Allergol Clin Immunol. 2012;22(1):20-7.