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Rat Brain PrimaCell™3: Normal Cerebral Vascular Endothelial Cells

The Rat Brain PrimaCell™ 3: Normal Cerebral Venous Vascular Endothelial Cells is for rapid (7-10 days) isolation and growth of normal rat cerebral venous vascular endothelial cells from newborn or adult rat brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Brain PrimaCell™ 3 System (Cat No. 2-82514):
Rat Brain PrimaCell™ 3: Normal Cerebral Venous Vascular Endothelial Cells Protocols
Rat Brain Tissue Preparation Buffer 3: Normal Cerebral Venous Vascular Endothelial Cells
Rat Brain OptiTDS™ 3: Tissue Dissociation System
Rat Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Rat Brain PrimaCell™ 3: Normal Cerebral Venous Vascular Endothelial Cells Growth Medium
Rat Brain PrimaCell™ 3: Normal Cerebral Venous Vascular Endothelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Tung HH, Lee SL. Neural transmembrane protease and endothelial Gs-protein activation in cell contact-dependent signaling between neural stem/progenitor cells and brain endothelial cells. J Biol Chem. 2012 May 10.
[2] Gauthier SA, Sahoo S, Jung SS, Levy E. Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain. Methods Mol Biol. 2012;849:261-74.
[3] Skinner RA, Gibson RM, Rothwell NJ, Pinteaux E, Penny JI. Transport of interleukin-1 across cerebromicrovascular endothelial cells. Br J Pharmacol. 2009 Apr;156(7):1115-23. Epub 2009 Mar 4.

Cat No. Description Unit Price Order
2-82514 Rat Brain PrimaCell™3: Normal Cerebral Vascular Endothelial Cells Kit $ 1039.50 
4-28221 Rat Brain OptiTDS™3: Tissue Dissociation System Kit $ 255.15 
7-15198 Rat Brain FibrOut™3: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 283.50 
9-25014 Rat Brain PrimaCell™3: Normal Cerebral Vascular Endothelial Cells Growth Medium 5 x 100 ml $ 122.85 
9-26014 Rat Brain PrimaCell™3: Normal Cerebral Vascular Endothelial Cells Growth Supplements with Serum (for 500 ml medium) set $ 283.50 
9-80221 Rat Brain Tissue Preparation Buffer 3: Normal Cerebral Vascular Endothelial Cells 1 x 100 ml $ 132.30 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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