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Rat Brain PrimaCell™9: Normal Neuron Cells

The Rat Brain PrimaCell™ 9: Normal Neuron Cells is for rapid (7-10 days) isolation and growth of normal rat neuron cells from newborn or adult rat brain tissues. This system features an optimal condition of tissue dissociation system, Brain OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Brain PrimaCell™ 9 System (Cat No. 2-82520):
Rat Brain PrimaCell™ 9: Normal Neuron Cells Protocols
Rat Brain Tissue Preparation Buffer 9: Normal Neuron Cells
Rat Brain OptiTDS™ 9: Tissue Dissociation System
Rat Brain FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Rat Brain PrimaCell™ 9: Normal Neuron Cells Growth Medium
Rat Brain PrimaCell™9: Normal Neuron Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Uliasz TF, Hamby ME, Jackman NA, Hewett JA, Hewett SJ. Generation of primary astrocyte cultures devoid of contaminating microglia. Methods Mol Biol. 2012;814:61-79.
[2] Tseng YT, Hsu YY, Shih YT, Lo YC. Paeonol attenuates microglia-mediated inflammation and oxidative stress-induced neurotoxicity in rat primary microglia and cortical neurons. Shock. 2012 Mar;37(3):312-8.
[3] Silva Bastos LF, Pinheiro de Oliveira AC, Magnus Schlachetzki JC, Fiebich BL. Minocycline reduces prostaglandin E synthase expression and 8-isoprostane formation in LPS-activated primary rat microglia. Immunopharmacol Immunotoxicol. 2011 Sep;33(3):576-80. Epub 2011 Jan 12.

Cat No. Description Unit Price Order
2-82520 Rat Brain PrimaCell™9: Normal Neuron Cells Kit $ 1039.50 
4-28227 Rat Brain OptiTDS™9: Tissue Dissociation System Kit $ 255.15 
7-15204 Rat Brain FibrOut™9: Fibroblast Inhibitory System (for 500 ml medium) Kit $ 283.50 
9-25020 Rat Brain PrimaCell™9: Normal Neuron Cells Growth Medium 5 x 100 ml $ 122.85 
9-26020 Rat Brain PrimaCell™9: Normal Neuron Cell Growth Supplements with Serum (for 500 ml medium) set $ 283.50 
9-80227 Rat Brain Tissue Preparation Buffer 9: Normal Neuron Cells 1 x 100 ml $ 132.30 
Ordering Information
Email:order@chiscientific.com
Tel:800.986.6008
Fax:978.897.5462
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