The Rat Eye PrimaCell™ 4: Normal Lens Epithelial Cells is for rapid (7-10 days) isolation and growth of normal Lens Epithelial Cells from rat eye tissues. This system features an optimal condition of tissue dissociation system, Eye OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Eye PrimaCell™ 4 System (Cat No. 2-82541):
Rat Eye PrimaCell™ 4: Normal Lens Epithelial Cells Protocols
Rat Eye Tissue Preparation Buffer 4: Normal Lens Epithelial Cells
Rat Eye OptiTDS™ 4: Tissue Dissociation System
Rat Eye FibrOut™ 4: Fibroblast Inhibitory System (for 500 ml medium)
Rat Eye PrimaCell™ 4: Normal Lens Epithelial Cells Growth Medium
Rat Eye PrimaCell™ 4: Normal Lens Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Okuno T, Nakanishi-Ueda T, Ueda T, Yasuhara H, Koide R. Ultraviolet Action Spectrum for Cell Killing of Primary Porcine Lens Epithelial Cells. J Occup Health. 2012 Mar 12.
[2] Schwirtlich M, Kwakowsky A, Emri Z, Antal K, Lacza Z, Cselenyák A, Katarova Z, Szabó G. GABAergic signaling in primary lens epithelial and lentoid cells and its involvement in intracellular Ca2+ modulation. Cell Calcium. 2011 Oct;50(4):381-92. Epub 2011 Aug 5.
[3] Long AC, Bomser JA, Grzybowski DM, Chandler HL. All-trans retinoic Acid regulates cx43 expression, gap junction communication and differentiation in primary lens epithelial cells. Curr Eye Res. 2010 Aug;35(8):670-9.