The Rat Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells is for rapid (7-10 days) isolation and growth of normal intestinal mucosa epithelial cells from rat Intestine tissues. This system features an optimal condition of tissue dissociation system, Intestine OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Intestine PrimaCell™ 1 System (Cat No. 2-82552):
Rat Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells Protocols
Rat Intestine Tissue Preparation Buffer 1: Normal Intestinal Mucosa Epithelial Cells
Rat Intestine OptiTDS™ 1: Tissue Dissociation System
Rat Intestine FibrOut™ 1: Fibroblast Inhibitory System (for 500 ml medium)
Rat Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells Growth Medium
Rat Intestine PrimaCell™ 1: Normal Intestinal Mucosa Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Pan D, Das A, Liu D, Veazey RS, Pahar B. Isolation and characterization of intestinal epithelial cells from normal and SIV-infected rhesus macaques. PLoS One. 2012;7(1):e30247. Epub 2012 Jan 26.
[2] Lahar N, Lei NY, Wang J, Jabaji Z, Tung SC, Joshi V, Lewis M, Stelzner M, Martín MG, Dunn JC. Intestinal subepithelial myofibroblasts support in vitro and in vivo growth of human small intestinal epithelium. PLoS One. 2011;6(11):e26898. Epub 2011 Nov 17.
[3] Wilhelm A, Jahns F, Böcker S, Mothes H, Greulich KO, Glei M. Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells. Toxicol In Vitro. 2012 Feb;26(1):133-41. Epub 2011 Oct 21.