The Rat Stomach PrimaCell™: Normal Stomach Mucosa Epithelial Cells is for rapid (7-10 days) isolation and growth of normal stomach mucosa epithelial cells from rat Stomach tissues. This system features an optimal condition of tissue dissociation system, Stomach OptiTDS™ that yields 5-7 times of single cells more than most of the tissue dissociation protocols published in the literature. In addition, this system ensures a high viability of the target cells with improved gradient contained in the culture medium. With CHI's proprietary fibroblast inhibitory system, FibrOut™, cells are growing with minimal amount of the unwanted cells such as fibroblasts.

Rat Stomach PrimaCell™ System (Cat No. 2-82585):
Rat Stomach PrimaCell™: Normal Stomach Mucosa Epithelial Cells Protocols
Rat Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells
Rat Stomach OptiTDS™: Tissue Dissociation System
Rat Stomach FibrOut™: Fibroblast Inhibitory System (for 500 ml medium)
Rat Stomach PrimaCell™: Normal Stomach Mucosa Epithelial Cells Growth Medium
Rat Stomach PrimaCell™: Normal Stomach Mucosa Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

References:

[1] Pan D, Das A, Liu D, Veazey RS, Pahar B. Isolation and characterization of intestinal epithelial cells from normal and SIV-infected rhesus macaques. PLoS One. 2012;7(1):e30247. Epub 2012 Jan 26.
[2] Lahar N, Lei NY, Wang J, Jabaji Z, Tung SC, Joshi V, Lewis M, Stelzner M, Martín MG, Dunn JC. Stomach subepithelial myofibroblasts support in vitro and in vivo growth of human small intestinal epithelium. PLoS One. 2011;6(11):e26898. Epub 2011 Nov 17.
[3] Wilhelm A, Jahns F, Böcker S, Mothes H, Greulich KO, Glei M. Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells. Toxicol In Vitro. 2012 Feb;26(1):133-41. Epub 2011 Oct 21.